Insulin binds to its receptor it forms this insulin receptor substrates and the next step is phosphorylation of AKT.
胰島素和胰島素受體聯(lián)系在一起形成了胰島素受體基質(zhì)下一步就是蘇氨酸蛋白激酶的氧化磷酸化過程
Which is a crucial step in bringing glucose transporters near the membrane and helping bring glucose inside the cell.
最重要的一步是將葡萄糖運轉(zhuǎn)體放在靠近細胞膜的位置然后把葡萄糖運送到細胞內(nèi)部
So what we measure here is the phosphorylation of AKT these are immunoblots.
所以我們這里測量AKT的氧化磷酸化情況這是免疫墨點法得出的結(jié)果
This is the insulin that every and all the adipocytes are exposed to increasing insulin concentration Total AKT is the control.
這里是胰島素所有脂肪細胞都面對胰島素濃度的升高AKT總量是控制
And you see, for example, on top here that the first blot that is clearly aberrant is at the concentration of 0.5 Nano molar of insulin.
例如 在最上面的部分第一個墨點顯然就表現(xiàn)出了異常胰島素濃度為0.5納摩爾
And then, I'm not going to blabber that but you can see that it takes a larger amount of insulin.
我不打算就這個指標(biāo)多講但大家可以看出當(dāng)試驗參與者的睡眠被剝奪時
To phosphorylate the same amount of AKT when the subject is sleep deprived and this was observed in each and every subject.
他們需要更多的胰島素才能對等量的AKT進行氧化磷酸化這種情況出現(xiàn)在每個試驗參與者身上
There were actually 7 subjects in this experiment which is coming out next...in two weeks from now where you see that the dose response curve of insulin....
實際有7名志愿者參與了這項試驗試驗將會在兩周后結(jié)束大家到時候可以看到胰島素的劑量反應(yīng)曲線
Of phosphorylation of AKT to insulin concentration is clearly shifted to the right so the adipocyte has become insulin resistance.
AKT的氧化磷酸化的劑量反應(yīng)曲線胰島素濃度曲線顯然會向右邊偏移因此脂肪細胞出現(xiàn)了胰島素抗性
And the change is enormous because the insulin concentration for half maximum response is basically doubled.
這種變化是巨大的因為半全反應(yīng)的胰島素濃度基本上翻了一番
You need twice as much as insulin to phosphorylate the same amount of AKT so that is one thing.
你需要兩倍的胰島素才能對等量的AKT進行氧化磷酸化處理這是一點
It's really demonstrative an important impact of sleep deprivation on the adipocyte my interest in this, it really is.
很說明問題顯示了睡眠剝奪對脂肪細胞的重大影響我所感興趣的是