評論
打印
PCR confirmation was performed on every sample
聚合酶鏈式反應(yīng)PCR被用于每個樣本
The study site was at 13 villages under jurisdiction of 4 health establishments
研究位置包括13個村分屬4個健康設(shè)施轄區(qū)
And these are located in one of the main focus of transmission in the Peruvian Amazon, It is here, okay
這些都位于秘魯亞馬遜地區(qū)主要傳播地，這里
We use 2 techniques for genotyping
我們使用了兩種基因型分析技術(shù)
The first one is PCR-RFLP technique which means that the primary product obtain it from PCR amplification here
第一種是PCR-RFLP技術(shù)，最初產(chǎn)品通過PCR擴增獲得
where then digest it using 2 restriction enzymes and then we got to profiles
然后兩種限制性內(nèi)切酶進行消化得到DNA片段
The second type of technique was, we use 9 different variable-number tandem repeat markers located in that area
第二種技術(shù)我們使用了該區(qū)域9種不同的可變數(shù)目串聯(lián)重復序列標記
including this certain merozoite surface protein in which we now is under selective pressure
包括這個裂殖子表面蛋白，我們知道它處在選擇壓力下
So by comparing additional type of these loci together at baseline
通過對比這些基因位點在基線
and at the recurrent sample relapses can be distinguished reliably from re-infections
及在再發(fā)作中的情況，復發(fā)能夠可靠地同再感染區(qū)分開來
For that analysis, we consider relapse if both were identical
分析中如果兩者相同，則認為是復發(fā)
and re-infection if at least for one of the markers we use it was different
如果其中一個標記不同，則認為是再感染
